antibody egfp origene goat polyclonal antibody  (OriGene)

Journal: Nature Communications

Article Title: Radial glia integrin avb8 regulates cell autonomous microglial TGFβ1 signaling that is necessary for microglial identity

doi: 10.1038/s41467-025-57684-y

Figure Lengend Snippet: Mouse Line and Reagent List

Article Snippet: Antibody , EGFP , Origene , Goat polyclonal antibody. Cat#: R1091P. RRID:AB_1002036. , Used at 1:2000..

Techniques: RNAscope, Multiplex Assay

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The in vitro under-agarose assay applied to PGCs. To apply moderate pressure to PGCs, the polystyrene beads are placed with PGCs between the gel and dish. Several large blebs are induced. b A PGC in which the blebs (white arrowheads) are induced by this assay. mCherry is overexpressed. The cell outline is delineated by a white dotted line. c Motion captures from the movie (Supplementary Movie 1) corresponding to the bleb region (white arrowheads) in GCaMP6s (green)-expressing PGC during a typical bleb cycle. d Changes in GCaMP6s fluorescence intensities in the cytoplasm of the bleb regions ( N =3) and cell bodies ( N =3) during a bleb cycle. E and R indicate extending and retracting phases of bleb formation, respectively. Error bars indicate standard error. GCaMP6s fluorescence intensity in each region is corrected by fluorescence intensity of co-introduced cytoplasmic mCherry. e GCaMP6s fluorescence intensity ratio in the bleb and cell body under the in vitro under-agarose assay. N =10. ***p < 0.001 (Two-sided, unpaired Student’s t test). f, g Motion captures from the movie (Supplementary Movie 2, 3) corresponding to the bleb region in MRLC-EGFP (green in f )- or EGFP-CAAX + Lifeact-mCherry (green and red in g , respectively)-expressing PGC during a typical bleb cycle. Yellow and white arrowheads indicate the extending and retracting bleb, respectively. Scale bars: 2.5 μm in b , c , 3 μm in f .

Article Snippet: After blocking, the sections were incubated at 4°C overnight using anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: In Vitro, Expressing, Fluorescence

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The distribution pattern of EGFP-CAAX (green) and Lifeact-mCherry (red) in a migrating PGC under the in vitro migration assay with SCF. PM, plasma membrane, b Motion captures from the movie (Supplementary Movie 7) about a GCaMP6s-expressing migrating PGC under the condition with SCF. c, d Ratio of Ca 2+ concentration (GCaMP6s fluorescence) at the front and rear end in migrating PGCs ( N =10 cells. 20 measurements per cell) and ratio of protrusion-forming PGCs ( N =30 cells) under the condition with/without SCF and SKF96365. Error bars indicate the standard error. e Motion captures from the movie (Supplementary Movie 8) taking a PGC stained by an ER tracker, ERseeing (green). The cell outline is delineated by a white dotted line. f Summary of PGC migration trajectory in the condition with Cos7 expressing SCF and SKF96365. N =15 cells. g Migration distances of PGCs for 30 min under the condition with/without SCF and SKF96365. N =15. Yellow arrowhead indicates the bleb formed at the cell front. ***: P<0.001 (Two-sided, unpaired Student’s t test). Scale bars: 10 μm in a, b, e.

Article Snippet: After blocking, the sections were incubated at 4°C overnight using anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: In Vitro, Migration, Clinical Proteomics, Membrane, Expressing, Concentration Assay, Fluorescence, Staining

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The pictures of EGFP (green) and Orai1 E106Q-overexpressing PGCs under the in vitro under-agarose assay. The cell outline is delineated by a white dotted line. b Ratio of bleb area to total cell area under the in vitro under-agarose assay. N =5 cells. c, d Summary of migration trajectories ( N =12 cells) and migration distances for 30 min ( N =12 cells) about PGCs expressing EGFP or Orai1 E106Q with EGFP under the in vitro migration assay with SCF. *: P<0.05, P<0.001 (Two-sided, unpaired Student’s t test). Scale bars: 5 μm in a.

Article Snippet: After blocking, the sections were incubated at 4°C overnight using anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: In Vitro, Migration, Expressing

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The distribution patterns of mCherry+ and Orai1 E106Q+ (also EGFP+) PGCs at HH 16 chick embryo after 5 hrs after infusion. Each is back-infused with 2,500 PGCs. Fluorescent images correspond to a square (Ex-VaP region) in the BF (bright field) image. b Quantification of arrested PGCs in Ex-VaP 5 hrs after infusion with 2,500 PGCs each. The total area occupied by each PGC line is calculated. c 3D reconstitution image of mCherry+ PGCs (blue) and EGFP (green) of Ex-VaP in HH16 quail embryo 6hrs after infusion. The vascular network of the embryo is immunostained by QH1 (red) and 3D-reconstracted. Left and right images are ventral and transverse view of Ex-VaP region, respectively. White arrows indicate transmigrating PGCs. White asterisks indicate remaining PGCs in intravascular space. d Ratio of transmigration rate of Orai1 E106Q+ PGCs. The number of EGFP+ (control) PGCs and Orai1 E106Q+ PGCs during and after transmigration is divided by the number of co-infused mCherry+ (infusion control) PGCs during and after transmigration, respectively. N =4 embryos. e The horizontal sections of E4.5 chicken embryos (HH25) 2 days after infusion with EGFP+ PGCs (green), Orai1 E106Q+ PGCs (green), and co-infused mCherry+ PGCs (red). White and yellow dotted lines delineate the dorsal mesenteric regions (DM) and gonadal primordium (G), respectively. f Ratio of the number of EGFP+ and Orai1 E106Q+ PGCs located in the DM and G 2 days after infusion. It is corrected by the number of co-infused mCherry+ PGCs. N =4 embryos. X and error bars indicate the mean value and standard error, respectively. **: P<0.01, ***: P<0.001 (Two-sided, unpaired Student’s t test). Scale bars: 1000 μm in a , 100 μm and 20 μm in left and right images of c , respectively, 100 μm in e .

Article Snippet: After blocking, the sections were incubated at 4°C overnight using anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: Transmigration Assay, Control

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The in vitro under-agarose assay applied to PGCs. To apply moderate pressure to PGCs, the polystyrene beads are placed with PGCs between the gel and dish. Several large blebs are induced. b A PGC in which the blebs (white arrowheads) are induced by this assay. mCherry is overexpressed. The cell outline is delineated by a white dotted line. c Motion captures from the movie (Supplementary Movie 1) corresponding to the bleb region (white arrowheads) in GCaMP6s (green)-expressing PGC during a typical bleb cycle. d Changes in GCaMP6s fluorescence intensities in the cytoplasm of the bleb regions ( N =3) and cell bodies ( N =3) during a bleb cycle. E and R indicate extending and retracting phases of bleb formation, respectively. Error bars indicate standard error. GCaMP6s fluorescence intensity in each region is corrected by fluorescence intensity of co-introduced cytoplasmic mCherry. e GCaMP6s fluorescence intensity ratio in the bleb and cell body under the in vitro under-agarose assay. N =10. ***p < 0.001 (Two-sided, unpaired Student’s t test). f, g Motion captures from the movie (Supplementary Movie 2, 3) corresponding to the bleb region in MRLC-EGFP (green in f )- or EGFP-CAAX + Lifeact-mCherry (green and red in g , respectively)-expressing PGC during a typical bleb cycle. Yellow and white arrowheads indicate the extending and retracting bleb, respectively. Scale bars: 2.5 μm in b , c , 3 μm in f .

Article Snippet: The blocked samples were treated at 4°C overnight with QH1 antibody (Hybridoma Bank, AB_531829, 1:200), anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: In Vitro, Expressing, Fluorescence

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The distribution pattern of EGFP-CAAX (green) and Lifeact-mCherry (red) in a migrating PGC under the in vitro migration assay with SCF. PM, plasma membrane, b Motion captures from the movie (Supplementary Movie 7) about a GCaMP6s-expressing migrating PGC under the condition with SCF. c, d Ratio of Ca 2+ concentration (GCaMP6s fluorescence) at the front and rear end in migrating PGCs ( N =10 cells. 20 measurements per cell) and ratio of protrusion-forming PGCs ( N =30 cells) under the condition with/without SCF and SKF96365. Error bars indicate the standard error. e Motion captures from the movie (Supplementary Movie 8) taking a PGC stained by an ER tracker, ERseeing (green). The cell outline is delineated by a white dotted line. f Summary of PGC migration trajectory in the condition with Cos7 expressing SCF and SKF96365. N =15 cells. g Migration distances of PGCs for 30 min under the condition with/without SCF and SKF96365. N =15. Yellow arrowhead indicates the bleb formed at the cell front. ***: P<0.001 (Two-sided, unpaired Student’s t test). Scale bars: 10 μm in a, b, e.

Article Snippet: The blocked samples were treated at 4°C overnight with QH1 antibody (Hybridoma Bank, AB_531829, 1:200), anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: In Vitro, Migration, Clinical Proteomics, Membrane, Expressing, Concentration Assay, Fluorescence, Staining

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The pictures of EGFP (green) and Orai1 E106Q-overexpressing PGCs under the in vitro under-agarose assay. The cell outline is delineated by a white dotted line. b Ratio of bleb area to total cell area under the in vitro under-agarose assay. N =5 cells. c, d Summary of migration trajectories ( N =12 cells) and migration distances for 30 min ( N =12 cells) about PGCs expressing EGFP or Orai1 E106Q with EGFP under the in vitro migration assay with SCF. *: P<0.05, P<0.001 (Two-sided, unpaired Student’s t test). Scale bars: 5 μm in a.

Article Snippet: The blocked samples were treated at 4°C overnight with QH1 antibody (Hybridoma Bank, AB_531829, 1:200), anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: In Vitro, Migration, Expressing

Journal: bioRxiv

Article Title: The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

doi: 10.1101/2023.06.12.544577

Figure Lengend Snippet: a The distribution patterns of mCherry+ and Orai1 E106Q+ (also EGFP+) PGCs at HH 16 chick embryo after 5 hrs after infusion. Each is back-infused with 2,500 PGCs. Fluorescent images correspond to a square (Ex-VaP region) in the BF (bright field) image. b Quantification of arrested PGCs in Ex-VaP 5 hrs after infusion with 2,500 PGCs each. The total area occupied by each PGC line is calculated. c 3D reconstitution image of mCherry+ PGCs (blue) and EGFP (green) of Ex-VaP in HH16 quail embryo 6hrs after infusion. The vascular network of the embryo is immunostained by QH1 (red) and 3D-reconstracted. Left and right images are ventral and transverse view of Ex-VaP region, respectively. White arrows indicate transmigrating PGCs. White asterisks indicate remaining PGCs in intravascular space. d Ratio of transmigration rate of Orai1 E106Q+ PGCs. The number of EGFP+ (control) PGCs and Orai1 E106Q+ PGCs during and after transmigration is divided by the number of co-infused mCherry+ (infusion control) PGCs during and after transmigration, respectively. N =4 embryos. e The horizontal sections of E4.5 chicken embryos (HH25) 2 days after infusion with EGFP+ PGCs (green), Orai1 E106Q+ PGCs (green), and co-infused mCherry+ PGCs (red). White and yellow dotted lines delineate the dorsal mesenteric regions (DM) and gonadal primordium (G), respectively. f Ratio of the number of EGFP+ and Orai1 E106Q+ PGCs located in the DM and G 2 days after infusion. It is corrected by the number of co-infused mCherry+ PGCs. N =4 embryos. X and error bars indicate the mean value and standard error, respectively. **: P<0.01, ***: P<0.001 (Two-sided, unpaired Student’s t test). Scale bars: 1000 μm in a , 100 μm and 20 μm in left and right images of c , respectively, 100 μm in e .

Article Snippet: The blocked samples were treated at 4°C overnight with QH1 antibody (Hybridoma Bank, AB_531829, 1:200), anti-EGFP goat polyclonal antibody (GeneTex, GTX266673, 1:1,000), and anti-mRFP rabbit polyclonal antibody (ROCKLAND, 600-401-379, 1:1,000) in the blocking solution.

Techniques: Transmigration Assay, Control

Journal: Scientific Reports

Article Title: Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo

doi: 10.1038/s41598-021-82752-w

Figure Lengend Snippet: Key resources. Information includes species and strains of animals used in this study, software, kits, antibodies, and reagents.

Article Snippet: Antibody , Anti-EGFP (goat polyclonal) , Origene , Origene:TA150095 , (1:2000).

Techniques: Software, Imaging, Labeling, Isolation, Recombinant, Plasmid Preparation